Proteins at Solid-Liquid Interfaces

✍ Scribed by Paul R. Van Tassel (auth.), Dr. Philippe Déjardin (eds.)

• Publisher: Springer-Verlag Berlin Heidelberg
• Year: 2006
• Tongue: English
• Leaves: 340
• Series: Principles and Practice
• Edition: 1
• Category: Library

✦ Synopsis

This book opens with a description of fundamental aspects of protein adsorption to surfaces, a phenomenon that plays a key role in biotechnological applications, especially at solid-liquid interfaces. Here are presented methods for studying adsorption kinetics and conformational changes such as optical waveguide lightmode spectroscopy (OWLS).

Also described are sensitive bench techniques for measuring the orientation and structure of proteins at solid-liquid interfaces, including total internal reflection ellipsometry (TIRE), dual polarisation interferometry (DPI) and time of flight - secondary ion mass spectrometry (TOF–SIMS).

A model study of fibronectin at polymer surfaces is included, as are studies using microporous membranes and textiles with immobilized enzymes for large-scale applications. Biocompatibility, anti-fouling properties and surface modification to modulate the adsorption and activity of biomolecules are among the other topics addressed in this useful and practical book.

✦ Table of Contents

Protein Adsorption Kinetics: Influence of Substrate Electric Potential....Pages 1-22
From Kinetics to Structure: High Resolution Molecular Microscopy....Pages 23-49
Initial Adsorption Kinetics in a Rectangular Thin Channel, and Coverage-Dependent Structural Transition Observed by Streaming Potential....Pages 51-73
Dual Polarisation Interferometry: An Optical Technique to Measure the Orientation and Structure of Proteins at the Solid-Liquid Interface in Real Time....Pages 75-104
Total Internal Reflection Ellipsometry: Monitoring of Proteins on Thin Metal Films....Pages 105-118
Conformations of Proteins Adsorbed at Liquid-Solid Interfaces....Pages 119-150
Evaluation of Proteins on Bio-Devices....Pages 151-173
Fibronectin at Polymer Surfaces with Graduated Characteristics....Pages 175-198
Development of Chemical Microreactors by Enzyme Immobilization onto Textiles....Pages 199-244
Approaches to Protein Resistance on the Polyacrylonitrile-based Membrane Surface: an Overview....Pages 245-269
Modulation of the Adsorption and Activity of Protein/Enzyme on the Polypropylene Microporous Membrane Surface by Surface Modification....Pages 271-298
Nonbiofouling Surfaces Generated from Phosphorylcholine-Bearing Polymers....Pages 299-326

✦ Subjects

Biotechnology;Biophysics/Biomedical Physics;Physical Chemistry;Biochemistry, general;Surfaces and Interfaces, Thin Films