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Protein–protein recognition: a computational mutagenesis study of the MDM2–P53 complex

✍ Scribed by Irina S. Moreira; Pedro A. Fernandes; Maria J. Ramos


Book ID
105886210
Publisher
Springer
Year
2008
Tongue
English
Weight
571 KB
Volume
120
Category
Article
ISSN
1432-2234

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## Abstract We report that MDM2, a negative regulator of p53, can bind to EBNA‐5. Using GST pull‐down assay, immunoprecipitation, surface plasmon resonance and immunostaining of lymphoblastoid cells, we found that trimolecular complexes are formed between EBNA‐5, MDM2 and p53, where MDM2 serves as