Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein
✍ Scribed by Cabantous, Stéphanie; Terwilliger, Thomas C; Waldo, Geoffrey S
- Book ID
- 109908326
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 340 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1044
No coin nor oath required. For personal study only.
📜 SIMILAR VOLUMES
When focused through an objective lens with a high numerical aperture, nanosecond pulses of high-intensity green (532-nm) laser light can be used to selectively destroy any cellular component whose boundaries can be defined by light microscopy. These components include, for example, chromosomes, spi
The adsorption of his-tag green fluorescent protein (GFPH 6 ) onto the mica surfaces has been studied by atomic force microscopy (AFM) and laser confocal fluorescence microscopy. By controlling the adsorption conditions, separated single GFPH 6 and GFPH 6 monolayer can be adsorbed and formed on mica