✦ LIBER ✦

Assembling and imaging of his-tag green fluorescent protein on mica surfaces studied by atomic force microscopy and fluorescence microscopy

✍ Scribed by Zhiguo Liu; Yuangang Zu; Yujie Fu; Zhonghua Zhang; Ronghua Meng

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 774 KB
Volume: 71
Category: Article
ISSN: 1059-910X
DOI: 10.1002/jemt.20622

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✦ Synopsis

The adsorption of his-tag green fluorescent protein (GFPH 6 ) onto the mica surfaces has been studied by atomic force microscopy (AFM) and laser confocal fluorescence microscopy. By controlling the adsorption conditions, separated single GFPH 6 and GFPH 6 monolayer can be adsorbed and formed on mica surfaces. In present experiments, based on the AFM measurement, we found that the adsorbed GFPH 6 was bound on the mica surface with its b-sheets. The formed GFPH 6 monolayer on mica surfaces was flat, uniform, and stable. Some applications of the formed monolayer have been demonstrated. The formed monolayer can be used as a substrate for DNA imaging and AFM mechanical lithography.

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## Abstract Transfer of a fluorescently labeled protein particle from a surface to a microsized scanning probe has been induced by repetitive scanning in aqueous medium. The so‐attached particle can in turn act as a probing tool to study particle–substrate and particle–particle interactions. Attach