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Protein synthesis in transformed 3T3 cells permeabilized by exogenous ATP

✍ Scribed by Takayuki Kitagawa

Publisher: John Wiley and Sons
Year: 1980
Tongue: English
Weight: 529 KB
Volume: 102
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041020106

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✦ Synopsis

Abstract

Exogenous ATP has been shown to cause a rapid and reversible increase in permeability in transformed 3T3 cells (3T6 and SV3T3) but not in untransformed 3T3 cells. The cells remain viable, but lose intracellular acid‐soluble pools. Treatment of transformed cells with ATP greatly reduces incorporation of ^14^C‐leucine into protein, which is restored by the incubation of the cells with Dulbecco's modified Eagle's medium or by the external additions of certain ions and energy sources. tRNA is not required for the restoration of protein synthesis. In the permeabilized cells the energy for protein synthesis can be provided by glycolysis, oxidative phosphorylation, or direct addition of ATP. These studies demonstrate the usefulness of this method for studying the control of metabolism and macromolecular synthesis in monolayer cultures of transformed mammalian cells.

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