Cottingen, Federal Republic of Germany Characteristics of glutamine transport, its substrate specificity, and its pattern of competitive and non-competitive inhibition in response to amino acid analogues were determined in peripheral human lymphocytes, incubated with or without concanavalin A (Con A
Protein synthesis in resting and stimulated human lymphocytes
β Scribed by Oscar Burrone; Israel D. Algranati
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 562 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0300-8177
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## Abstract Resting lymphocytes are in the G~0~ phase of the cell cycle. Upon activation by PHA, they progress into G~1~ with accompanying increased protein and RNA synthesis, initiate DNA synthesis and divide. We have studied the kinetics of inhibition of macromolecular synthesis during activation
The effect of malathion, an organophosphorus insecticide, on DNA and RNA synthesis was investigated by measuring the rate of incorporation of 3H thymidine and 3H uridine, respectively, into human lymphocytes stimulated by phytohemagglutinin (PHA). Increasing concentrations of malathion, from 10 to 7