๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Protein synthesis by blastocysts in the uteri and oviducts of intact and hypophysectomized mice

โœ Scribed by Weitlauf, Harry M.

Publisher
John Wiley and Sons
Year
1971
Tongue
English
Weight
447 KB
Volume
176
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

โœฆ Synopsis

Abstract

Two possible explanations for the stimulatory effect of estrogen and progesterone on protein synthesis by preimplantation mouse embryos are that the hormones: (1) stimulate the embryos directly; or (2) induce changes in the uterus that in turn affect the embryos.

To determine which of these possibilities is correct, mouse blastocysts were retained in one oviduct by a ligature at the uterotubal junction; the mice were then either left intact or hypophysectomized. ^35^S methionine was injected and the tubal and uterine blastocysts were recovered and prepared for radioautography.

Blastocysts in utero incorporate ^35^S methionine only if the pituitary is present. On the other hand, blastocysts in the oviducts incorporate ^35^S methionine regardless of the hormonal status of the mother. This suggests that estrogen and progesterone influence the level of protein synthesis in uterine blastocysts by inducing changes in the uterus rather than by stimulating the embryos directly.

In addition, blastocysts were recovered from oviducts or uteri between day 5 and day 10 post coitum and transferred to pseudopregnant females to test their ability to develop into normal fetuses. Uterine blastocysts retained the ability to develop normally while the percentage of tubal blastocysts capable of development dropped sharply between day 5 and day 10. Therefore, it is suggested that the dormant condition of mouse blastocysts in utero during delayed implantation may serve to protect the embryos from damage.

๐Ÿ“œ SIMILAR VOLUMES

In vitro uptake and incorporation of ami
In vitro uptake and incorporation of amino acids by blastocysts from intact and ovariectomized mice
โœ Weitlauf, Harry M. ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 449 KB ๐Ÿ‘ 1 views

## Abstract Blastocysts were recovered from the uteri of intact or ovariectomized mice and incubated with a mixture of ^14^C amino acids in vitro. The level of uptake and incorporation of the amino acids as estimated by scintillation counting was higher in blastocysts from intact than in those from

Gastroschisis is caused by the combinati
Gastroschisis is caused by the combination of carbon monoxide and protein-zinc deficiencies in mice
โœ Jarnail Singh ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 101 KB

## Abstract **BACKGROUND:** Gastroschisis is a rare congenital defect of the abdominal wall. Its occurrence is noted primarily in the offspring of young mothers who often smoke during pregnancy. The incidence of gastroschisis has been increasing in many countries in recent years. The etiology of ga

Effect of microwaves (2450-MHz) on the i
Effect of microwaves (2450-MHz) on the immune system in mice: Studies of nucleic acid and protein synthesis
โœ Dr. W. Wiktor-Jedrzejczak; A. Ahmed; P. Czerski; W. M. Leach; K. W. Sell ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 548 KB

## Abstract CBA/J adult male mice were given single or triple exposures to 2450โ€MHz microwaves in an environmentally controlled wave guide facility. The average absorbed dose rate for a single exposure varied from 12 to 15 mW/g. Shamexposed mice served as controls. Lymphoid cells were collected and