Protein sequencing by mass analysis of polypeptide ladders after controlled protein hydrolysis
β Scribed by Zhong, Hongying; Zhang, Ying; Wen, Zhihui; Li, Liang
- Book ID
- 109908294
- Publisher
- Nature Publishing Group
- Year
- 2004
- Tongue
- English
- Weight
- 456 KB
- Volume
- 22
- Category
- Article
- ISSN
- 1087-0156
- DOI
- 10.1038/nbt1011
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## Abstract Under favorable conditions, Asp or Asn residues can undergo rearrangement to a succinimide (cyclic imide), which may also serve as an intermediate for deamidation and/or isoaspartate formation. Direct identification of such succinimides by peptide mapping is hampered by their lability a