## Abstract An integrated platform with the combination of protein and peptide separation was established via online protein digestion, by which proteins were first separated by CIEF, online digested by a trypsin immobilized enzyme microreactor, trapped and desalted by two parallel trap columns, se
Protein profiling by capillary isoelectric focusing, reversed-phase liquid chromatography, and mass spectrometry
✍ Scribed by Feng Zhou; Murray V. Johnston
- Book ID
- 102183973
- Publisher
- John Wiley and Sons
- Year
- 2005
- Tongue
- English
- Weight
- 484 KB
- Volume
- 26
- Category
- Article
- ISSN
- 0173-0835
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## Abstract A comprehensive two‐dimensional (2‐D) separation system, coupling capillary reverse‐phase liquid chromatography (cRPLC) to capillary isoelectric focusing (CIEF), is described for protein and peptide mapping. cRPLC, the first dimension, provided high‐resolution separations for salt‐free