On-line capillary isoelectric focusing CIEF ᎐electrospray ionization Ž . time-of-flight mass spectrometry ESI᎐TOFMS as a two-dimensional separation Ž system is employed for high-resolution analysis of model proteins myoglobin and . -lactoglobulin and human hemoglobin variants C, S, F, and A. The fo
Capillary isoelectric focusing–mass spectrometry: analysis of protein mixtures from human body fluids
✍ Scribed by Nigel J. Clarke; Stephen Naylor
- Publisher
- John Wiley and Sons
- Year
- 2002
- Tongue
- English
- Weight
- 211 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0269-3879
- DOI
- 10.1002/bmc.159
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Capillary isoelectric focusing (CIEF) can provide high-resolution separations of complex protein mixtures, but until recently it has primarily been used with conventional UV detection. This technique would be greatly enhanced by much more information-rich detection methods that can aid in protein ch
On-line capillary zone electrophoresis/electrospray ionization mass spectrometry (CZE-ESMS) and capillary isoelectric focusing/electrospray ionization mass spectrometry (CIEF/ESMS) were employed for protein analysis. The separation mechanisms and the detection limits of CZE/ESMS and CIEF/ESMS were c