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Protein phosphatase 2Aα involvement in granulocytic differentiation of HL-60 cells

✍ Scribed by Kazunori Sasaki; Kazuma Ikeda; Masami Nagai; Hiroshi Shima; Minako Nagao; Jiro Takahara; Shozo Irino

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
340 KB
Volume
11
Category
Article
ISSN
0278-0232

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✦ Synopsis

Protein phosphatase 2Aa (PP-2Aa) is one of the catalytic subunits of PP-2A, which is composed of catalytic and regulatory subunits. In fission yeasts, PP-2A mutants cause mitotic defects and strikingly different cell cycle phenotypes. In rat hepatocellular carcinomas, mRNA expression of PP-2Aa was increased compared to that of normal hepatocytes. From these studies, PP-2Aa has been thought to be involved in proliferation, and we expected that PP-2Aa would decrease in HL-60 cells on dimethyl sulfoxide(DMS0)-induced granulocytic differentiation with decreased growth rate. In this study, we show that PP-2Aa expression increases in HL-60 cells on DMSO-induced granulocytic differentiation, while PP-ly, which is one of catalytic subunits of protein phosphatase 1, expression did not change significantly. These results suggest that PP-2Aa may be involved in regulation of differentiation of hematopoietic cells.

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