✦ LIBER ✦

Elevation of a potassium current in differentiating human leukemic (HL-60) cells

✍ Scribed by Steven J. Wieland; Robin H. Chou; Thelma A. Chen

Publisher: John Wiley and Sons
Year: 1987
Tongue: English
Weight: 432 KB
Volume: 132
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041320227

No coin nor oath required. For personal study only.

✦ Synopsis

Human promyelocytic leukemia (HL-60) cells display a novel voltage-dependent outward current under voltage clamp. This current is present at low levels in the proliferative state and in granulocytes derived from HL-60 cells which were induced to differentiate with retinoic acid. It is elevated in macrophages derived from HL-60 cells after exposure to phorbol-12-myristate-13acetate (PMA). The current is carried primarily by K + , is blocked by Cs+ and by increased intracellular concentrations of CI-. From a holding potential of -80 mV, significant activation required depolarization to + 20 mV membrane

📜 SIMILAR VOLUMES

Macrophage-colony-stimulating factor (CS

Macrophage-colony-stimulating factor (CSF-1) modulates a differentiation-specific inward-rectifying potassium current in human leukemic (HL-60) cells

✍ Steven J. Wieland; Robin H. Chou; Qi-Hua Gong 📂 Article 📅 1990 🏛 John Wiley and Sons 🌐 English ⚖ 849 KB

I9 102 A voltage-activated inward-rectifying K + conductance (IK,) appears in human promyelocytic leukemia (HL-60) cells during phorbol ester-induced differentiation into macrophages. This conductance was detected in the cells 24 hours after exposure to phorbol-12-myristate-13-acetate (PMA), as the

Self-renewal and commitment to different

Self-renewal and commitment to differentiation of human leukemic promyelocytic cells (HL-60)

✍ Eitan Fibach; Tony Peled; Eliezer A. Rachmilewitz 📂 Article 📅 1982 🏛 John Wiley and Sons 🌐 English ⚖ 879 KB

## Abstract More than 80% of cells from a human promyelocytic leukemic cell line (HL‐60) possess the capacity for self‐renewal as evidenced by their ability to form large primary colonies in semisolid medium and the presence within these colonies of cells capable of subsequent colony formation. Col

Rapid changes in bidirectional K+ fluxes

Rapid changes in bidirectional K+ fluxes preceding DMSO-induced granulocytic differentiation of HL-60 human leukemic cells

✍ J. J. Gargus; E. A. Adelberg; C. W. Slayman 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 775 KB

## Abstract When grown in medium containing 5 mM potassium and 140 mM sodium, HL‐60, a human promyelocytic cell line, maintained a steady‐state intracellular K^+^ concentration of 145 mmol/L cells and a steady‐state intracellular Na^+^ concentration of 30 mmol/L cells. Nearly 90% of the unidirectio

Modulation of cytokine production by int

Modulation of cytokine production by interferential current in differentiated HL-60 cells

✍ W. Sontag 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 131 KB 👁 1 views

The influence of interferential current (IFC) on the release of four cytokines was investigated. IFC is an amplitude-modulated 4 kHz current used in therapeutic applications. Human promyelocytes (HL-60) were differentiated to monocytes/macrophages by treatment with calcitriol. Release of tumor necro

Magnesium restriction induces granulocyt

Magnesium restriction induces granulocytic differentiation and expression of P27Kip1 in human leukemic HL-60 cells

✍ Valeria Covacci; Nicodemo Bruzzese; Alessandro Sgambato; Arianna Di Francesco; M 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 314 KB 👁 2 views

When cultured in Mg restricted medium, human leukemic HL-60 cells develop morphological and functional granulocytic differentiation. In 0.03 mM Mg, cells display the distinctive features of differentiation, without appreciable inhibition of proliferation. In 0.01 mM Mg, cells show terminal different

Intranuclear distribution of the human m

Intranuclear distribution of the human myeloid cell nuclear differentiation antigen in HL-60 cells

✍ David M. Duhl; Marek Gaczynski; Ryszard Olinski; Robert C. Briggs 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 688 KB

Based on solubility properties, the human myeloid cell nuclear differentiation antigen exists as at least two distinct populations. Most is easily extracted from isolated nuclei in 0.35 M NaCI, while 20 percent resists such treatment. Compared to undigested nuclei, both the amount of myeloid cell nu