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Protein Lipidation Protocols Volume 116 || Preparation and Assay of Myristoyl-CoA:Protein N-Myristoyltransferase

✍ Scribed by Gelb, Michael H.

Book ID
121366922
Publisher
Humana Press
Year
1999
Weight
213 KB
Category
Article
ISBN-13
9781592592647

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πŸ“œ SIMILAR VOLUMES

An Assay for Myristoyl-CoA:Protein N-Myr
An Assay for Myristoyl-CoA:Protein N-Myristoyltransferase Activity Based on Ion-Exchange Exclusion of [3H] Myristoyl Peptide
✍ S.A. French; H. Christakis; R.R. Oneill; S.P.F. Miller πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 552 KB

We developed an assay to test for inhibition of myristoyl-CoA:protein \(\mathbf{N}\)-myristoyltransferase (NMT) activity since this enzyme is important in viral replication and cellular biochemistry. Saccharomyces cerevisae NMT was harvested from Escherichia coli carrying a plasmid vector containing