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Protein-lipid interactions: A study of (Ca2+-Mg2+)ATPase reconstituted with synthetic phospholipids

✍ Scribed by Juan C. Gómez-Fernández; Félix M. Goñi; Dina Bach; Colin Restall; Dennis Chapman

Book ID
115909473
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
469 KB
Volume
98
Category
Article
ISSN
0014-5793

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A detailed methodology is presented of two reconstitution protocols for the (Ca(2+)-Mg2+)-ATPase from rabbit skeletal muscle, using the detergent potassium cholate. Method A was shown to produce fully fragmented membranes of definable lipid to protein ratios, which were unable to take up calcium upo