✍ Scribed by Nienhaus, G. Ulrich
No coin nor oath required. For personal study only.
A Readily Reproducible Collection Of Established And Emerging Techniques For Studying The Interaction Between Proteins And Ligands, Including Biochemical/bulk Techniques, Structure Analysis, Spectroscopy, Single-molecule Studies, And Theoretical/computational Tools. Among The Highlights Are Surface Plasmon Resonance (spr) And Reflectometric Biosensor Approaches, High-throughput Screening With Confocal Optics Microscopy, Single Molecule Fluorescence And Fluorescence Correlation Spectroscopy (fcs), Atomic Force Microscopy (afm), Crystallography Of Reaction Intermediates, And Time-resolved X-ray Crystallography. The Protocols Follow The Successful Methods In Molecular Biologytm Series Format, Each Offering Step-by-step Laboratory Instructions, An Introduction Outlining The Principle Behind The Technique, Lists Of The Necessary Equipment And Reagents, And Tips On Troubleshooting And Avoiding Known Pitfalls.
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A Readily Reproducible Collection Of Established And Emerging Techniques For Studying The Interaction Between Proteins And Ligands, Including Biochemical/bulk Techniques, Structure Analysis, Spectroscopy, Single-molecule Studies, And Theoretical/computational Tools. Among The Highlights Are Surface