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Protein kinase C subspecies in estrogen receptor-positive and -negative human breast cancer cell lines

✍ Scribed by Eric Bignon; Kouji Ogita; Akira Kishimoto; Yasutomi Nishizuka

Book ID
115762956
Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
518 KB
Volume
171
Category
Article
ISSN
0006-291X

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## Abstract We show that flavonoids positively regulate type‐II estrogen‐binding site (type‐ll EBS) levels both in MCF‐7 (ER‐positive) and in MDA‐MB231 (ER‐negative) breast‐cancer cells. Type‐ll EBS were measured by a whole‐cell assay at 4°C for 2.5 hr using [^3^H]‐estradiol as tracer. In both cell