Protein kinase C isozymes signaling in the heart
β Scribed by Dipak K Das
- Book ID
- 117142393
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 54 KB
- Volume
- 35
- Category
- Article
- ISSN
- 0022-2828
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Understanding transmembrane signalling process is one of the major challenge of the decade. In most tissues, since Fisher and Krebs's discovery in the 1950's, protein phosphorylation has been widely recognized as a key event of this cellular function. Indeed, binding of hormones or neurotransmitters
A calcium-sensitive, phospholipid-dependent protein kinase (protein kinase C) and its three isozymes were purified from rat heart cytosolic fractions utilizing a rapid purification method. The purified protein kinase C enzyme showed a single polypeptide band of 80 KDa on SDS-polyacrylamide gel elect