Protein kinase C – A family of protein kinases, allosteric effectors or both?
✍ Scribed by Angus J.M. Cameron; Peter J. Parker
- Book ID
- 113418988
- Publisher
- Elsevier Science
- Year
- 2010
- Tongue
- English
- Weight
- 654 KB
- Volume
- 50
- Category
- Article
- ISSN
- 0065-2571
No coin nor oath required. For personal study only.
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We report a simple method that permits simultaneous detection of multiple protein kinase activities using postnuclear supernatant of \(\mathbf{v}-8\) src transformed NIH3T3 cells. A supernatant is incubated with activators of protein kinases and \(\left[\gamma-{ }^{32} \mathbf{P}\right] \mathrm{ATP}
## Homologies in amino-acid sequence indicate that all known protein kinases share a conserved catalytic core, and, thus, belong to a relatedfamily ofproteins that have evolved in part from a common ancestoral origin. This family includes cellular kinases, oncogenic viral kinases and their protoonco