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Protein identification from 2-DE gels by MALDI mass spectrometry

✍ Scribed by Peter Jungblut; Bernd Thiede

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 259 KB
Volume: 16
Category: Article
ISSN: 0277-7037
DOI: 10.1002/(sici)1098-2787(1997)16:3<145::aid-mas2>3.0.co;2-h

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✦ Synopsis

Two-dimensional electrophoresis (2-DE) allows the separation species shows the capacity of the combination of 2-DE with MALDI-MS. ᭧ 1997 John Wiley & Sons, Inc., Mass Spectrom of proteins at the level of protein species, which are defined by their chemical structure. Each chemical modification leads to a Rev 16(3), 145-162, 1997 new protein species. Prerequisites for investigations of protein species are reproducible sample preparation, large gels (20 1 30 cm and larger), high sensitive detection methods, automated have the potential for complete structural investigation on the ing progress. A comparable comprehensive analysis of proprotein species level. The discrimination of 19 crystalline species of mouse eye lens and of 54 human heart heatshock protein 27

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