𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Protein engineering as a tool for crystallography

✍ Scribed by Stephen R Price; Kiyoshi Nagai


Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
686 KB
Volume
6
Category
Article
ISSN
0958-1669

No coin nor oath required. For personal study only.


πŸ“œ SIMILAR VOLUMES


Attempts to engineer anEscherichia coliD
✍ R. Caswell; A. Lyddiatt; S. Busby; M. Downham πŸ“‚ Article πŸ“… 1993 πŸ› Springer-Verlag 🌐 English βš– 498 KB

Restriction sites were introduced into the Eschcrichia coli melR gene that facilitated the fusion of other proteins to MelR. Both R-galactosidase and the constant domain of the human IgG kappa light chain were fused to MelR. However, whilst unmodified MelR could be over-expressed, neither MelR fusio

Peptide Mapping of Subtilisins as a Prac
✍ T. Christianson; C. Paech πŸ“‚ Article πŸ“… 1994 πŸ› Elsevier Science 🌐 English βš– 735 KB

Protein engineering based on rational design is an iterative process of sequential amino acid residue replacements. This requires a rapid and sensitive method for checking the protein sequence after each round of mutagenesis. As shown with subtilisin BL, acid treatment followed by urea denaturation