Protein engineering as a tool for crystallography
β Scribed by Stephen R Price; Kiyoshi Nagai
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 686 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0958-1669
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Protein engineering based on rational design is an iterative process of sequential amino acid residue replacements. This requires a rapid and sensitive method for checking the protein sequence after each round of mutagenesis. As shown with subtilisin BL, acid treatment followed by urea denaturation