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Attempts to engineer anEscherichia coliDNA-binding protein as a tool for affinity purification of heterologous proteins

✍ Scribed by R. Caswell; A. Lyddiatt; S. Busby; M. Downham

Publisher: Springer-Verlag
Year: 1993
Tongue: English
Weight: 498 KB
Volume: 7
Category: Article
ISSN: 0951-208X
DOI: 10.1007/bf00150904

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✦ Synopsis

Restriction sites were introduced into the Eschcrichia coli melR gene that facilitated the fusion of other proteins to MelR. Both R-galactosidase and the constant domain of the human IgG kappa light chain were fused to MelR. However, whilst unmodified MelR could be over-expressed, neither MelR fusion protein was over-produced. Addition of an extra domain to MelR leads to reduced expression in a number of genetic backgrounds.

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