Preparative elution of proteins blotted
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Boguslaw Szewczyk; Donald F. Summers
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Article
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1988
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Elsevier Science
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English
⚖ 909 KB
Conditions for the preparative elution of proteins from Immobilon membranes after transfer of proteins to this matrix from sodium dodecyl sulfate-polyacrylamide gels have been established. Proteins were completely eluted from the membrane at room temperature by short incubation in 50 mM Tris-HCl, pH