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Protein elution and blotting by centrifugation

✍ Scribed by Leonila Fajardo Hermansen; Öyvind Pedersen; Knut Sletten


Book ID
105273990
Publisher
Springer
Year
1992
Tongue
English
Weight
103 KB
Volume
11
Category
Article
ISSN
1573-4943

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Preparative elution of proteins blotted
✍ Boguslaw Szewczyk; Donald F. Summers 📂 Article 📅 1988 🏛 Elsevier Science 🌐 English ⚖ 909 KB

Conditions for the preparative elution of proteins from Immobilon membranes after transfer of proteins to this matrix from sodium dodecyl sulfate-polyacrylamide gels have been established. Proteins were completely eluted from the membrane at room temperature by short incubation in 50 mM Tris-HCl, pH