Protein determination on an automatic spectrophotometer

✍ Scribed by Dennis B. Rylatt; Christopher R. Parish

• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 164 KB
• Volume: 121
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90578-4

✦ Synopsis

Rapid and sensitive estimations of protein can be. carried out in 96-well microplates in an automated spectrophotometer of the type developed for microenzyme linked immunosorbent assay determinations.

The procedure is based on the Coomassie blue method of J. J. Sedmark and S. E. Grossberg (1977, Anal. Biochem. 79, 544-552) and can detect less than 200 ng of bovine plasma albumin and results in up to a 50-fold reduction in processing time over that required with a conventional manual spectrophotometer.