Rapid and sensitive estimations of protein can be. carried out in 96-well microplates in an automated spectrophotometer of the type developed for microenzyme linked immunosorbent assay determinations. The procedure is based on the Coomassie blue method of J. J. Sedmark and S. E. Grossberg (1977, An
An automatic determination of protein-bound hexose
β Scribed by J. Judd; W. Clouse; J. Ford; J. van Eys; L.W. Cunningham
- Publisher
- Elsevier Science
- Year
- 1962
- Tongue
- English
- Weight
- 144 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0003-2697
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A very suitable spectrophotometric method for simultaneous determination of protein-bound hexoses and fucose is presented. A mixture of L-cysteine and phenol in sulfuric acid was used as reagent, whereas absorption measurements were carried out at two wavelengths, namely at 398 nm for fucose and 490
The application of an automatic image analyzer (TAS, Leitz, Wetzlar) for determination of labeling density in protein A-gold labeled sections is described. Electron micrographs of rat liver labeled with 12 nm gold particles for peroxisomal enzymes are placed on the macrounit of TAS and the images of