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Protein determination by Lowry's method in the presence of sulfhydryl reagents

โœ Scribed by P.J. Geiger; S.P. Bessman

Book ID
107711362
Publisher
Elsevier Science
Year
1972
Tongue
English
Weight
384 KB
Volume
49
Category
Article
ISSN
0003-2697

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Lowry determination of protein in the pr
Lowry determination of protein in the presence of sulfhydryl compounds or other reducing agents
โœ Masashi Higuchi; Fumio Yoshida ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 342 KB

The Lowry procedure has been modified for use in the presence of sulfhydryl compounds or other reducing agents by the addition of chloramine T under acid conditions before adding the alkaline-copper reagent. This method is applicable to the determination of 25-100 pg of protein, and a linear standar