Protein denaturation by combined effect of shear and air-liquid interface

✍ Scribed by Yuh-Fun Maa; Chung C. Hsu

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 383 KB
• Volume: 54
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/(sici)1097-0290(19970620)54:6<503::aid-bit1>3.0.co;2-n

✦ Synopsis

The effect of shear alone on the aggregation tem, it was concluded that neither high shear (Ͼ10 6 ) of recombinant human growth hormone (rhGH) and renor high shear rate (Ͼ10 5 sec Ϫ1 ) alone had a significant combinant human deoxyribonuclease (rhDNase) has effect on aggregation though it induced slight conformabeen found to be insignificant. This study focused tional changes and clipping on rhGH (Maa and Hsu, on the synergetic effect of shear and gas-liquid inter-1996a). In this study, the combined effect of shear and face on these two model proteins. Two shearing systems, the concentric-cylinder shear device (CCSD) and the air-liquid interface on proteins was investigated.

rotor/stator homogenizer, were used to generate high Protein adsorption is a well-known phenomenon that shear (Ͼ10 6 ) in aqueous solutions in the presence of affects a wide variety of processes involving two or more air. High shear in the presence of an air-liquid inter-