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Protein Characterization by On-Line Capillary Isoelectric Focusing, Reversed-Phase Liquid Chromatography, and Mass Spectrometry

✍ Scribed by Zhou, Feng; Johnston, Murray V.


Book ID
127079612
Publisher
American Chemical Society
Year
2004
Tongue
English
Weight
199 KB
Volume
76
Category
Article
ISSN
0003-2700

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## Abstract An integrated platform with the combination of protein and peptide separation was established via online protein digestion, by which proteins were first separated by CIEF, online digested by a trypsin immobilized enzyme microreactor, trapped and desalted by two parallel trap columns, se