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Protein array method for assessing in vitro biomaterial-induced cytokine expression

✍ Scribed by Yiwen Li; Robert J. Schutte; Amal Abu-Shakra; W.Monty Reichert

Book ID
108069369
Publisher
Elsevier Science
Year
2005
Tongue
English
Weight
359 KB
Volume
26
Category
Article
ISSN
0142-9612

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A quantitative in vitro amino acid incorporation assay is described which can be used to assess the status of in vivo protein synthesis. The preparation and incubation conditions employed result in constant precursor specific activity and limit amino acid incorporation to completion of nascent pepti