๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

An in vitro amino acid incorporation method for assessing the status of in vivo protein synthesis

โœ Scribed by Thaddeus S. Nowak Jr.; Elizabeth R. Carty; W.David Lust; Janet V. Passonneau

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
911 KB
Volume
136
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A quantitative in vitro amino acid incorporation assay is described which can be used to assess the status of in vivo protein synthesis. The preparation and incubation conditions employed result in constant precursor specific activity and limit amino acid incorporation to completion of nascent peptide chains. Results obtained with this method correlate well with measurements of polyribosome profiles using sucrose gradient centrifugation. The assay is easily applied to a large number of samples, and requires only a fraction of the time and tissue necessary for conventional measures of polysome aggregation. The method has been found suitable for studies of protein synthesis in mouse brain and liver, and in gerbil brain, but not in mouse kidney. Products of in vitro protein synthesis can be separated by standard electrophoretic techniques, allowing a characterization of proteins whose mRNAs are actively translated in vivo.

๐Ÿ“œ SIMILAR VOLUMES

The in vitro synthesis and processing of
The in vitro synthesis and processing of the branched-chain amino acid binding proteins
โœ Daniels, Charles J. ;Anderson, James J. ;Landick, Robert ;Oxender, Dale L. ๐Ÿ“‚ Article ๐Ÿ“… 1980 ๐Ÿ› Wiley (John Wiley & Sons) ๐ŸŒ English โš– 439 KB ๐Ÿ‘ 2 views

## Abstract The synthesis of the leucineโ€specific and LIVโ€binding proteins was examined in vitro in a coupled transcription/translation system using the hybrid plasmids pOX7 and pOX13 as templates. Plasmid pOX7 contains the __livK__ gene coding for the leucineโ€specific binding protein, and pOX13 co

Amino Acids, Peptides and Proteins in Or
Amino Acids, Peptides and Proteins in Organic Chemistry (Origins and Synthesis of Amino Acids) || Methods for the Chemical Synthesis of Noncoded รŽยฑ-Amino Acids found in Natural Product Peptides
โœ Hughes, Andrew B. ๐Ÿ“‚ Article ๐Ÿ“… 2010 ๐Ÿ› Wiley-VCH Verlag GmbH & Co. KGaA ๐ŸŒ German โš– 685 KB

This is the first of five books in the *Amino Acids, Peptides and Proteins in Organic Synthesis* series. Closing a gap in the literature, this is the only series to cover this important topic in organic and biochemistry. Drawing upon the combined expertise of the international "who's who" in amino