Protein and Genome Evolution in Mammalian Cells for Biotechnology Applications
β Scribed by Brian S. Majors; Gisela G. Chiang; Michael J. Betenbaugh
- Book ID
- 107581702
- Publisher
- Humana Press Inc
- Year
- 2009
- Tongue
- English
- Weight
- 421 KB
- Volume
- 42
- Category
- Article
- ISSN
- 1073-6085
No coin nor oath required. For personal study only.
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Most proteins for structural biology studies are produced by high-level expression in __Escherichia coli.__ However, prokaryotic based expression systems fail to generate correctly folded functional forms of many proteins and hence a variety of eukaryotic based expression systems have been developed
## Abstract There are a growing number of reports on the subβphysiological temperature culturing (<37Β°C) of mammalian cells for increased recombinant protein yield, although the effect is variable between cell lines, expression systems, and the product of interest. What is becoming clear is that ex