Protein alkylation by acrylamide, its N-substituted derivatives and cross-linkers and its relevance to proteomics: A matrix assisted laser desorption/ionization-time of flight-mass spectrometry study

Matrix-assisted laser desorption/ionisat

Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry for monitoring alkylation of B-lactoglobulin B exposed to a series of N-substituted acrylamide monomers

✍ Ellenia Bordini; Mahmoud Hamdan; Pier Giorgio Righetti 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 114 KB 👁 2 views

Delayed-extraction matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, in both linear and reflectron modes, has been used to examine the alkylation of bovine blactoglobulin-bound cysteines exposed to various molar concentrations (0.5-30 mM) of acrylamide and a n