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Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry for monitoring alkylation of B-lactoglobulin B exposed to a series of N-substituted acrylamide monomers

✍ Scribed by Ellenia Bordini; Mahmoud Hamdan; Pier Giorgio Righetti

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 114 KB
Volume: 13
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(19991115)13:21<2209::aid-rcm777>3.0.co;2-s

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✦ Synopsis

Delayed-extraction matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry, in both linear and reflectron modes, has been used to examine the alkylation of bovine blactoglobulin-bound cysteines exposed to various molar concentrations (0.5-30 mM) of acrylamide and a number of its N-substituted monomers. These measurements were conducted at pH $ 9.5, and showed that at 0.5 mM all monomers (except N-acryloylaminopropanol) resulted in a measurable alkylation of at least one cysteine out of five. At higher concentrations (15 mM) all investigated monomers resulted in substantial alkylation which, for some, involved all five cysteine residues. Reflectron MALDI-TOF measurements of a number of alkylated protein digests revealed that, at low molar ratios, the alkylation site is influenced by the identity of the monomer. For example acrylamide and N,N-dimethylacrylamide attacked Cys 160 as well as one of the three cysteines within the tryptic fragment [102-124], while other investigated monomers did not involve Cys 160 . The implications of the present data for two-dimensional (2D) gel electrophoresis, and their eventual correlation to the toxicity of the investigated monomers, are discussed.

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