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Protein 1: Its purification and application in clinical medicine

✍ Scribed by Dr. Yoshihisa Itoh; Shuichi Ishii; Ryuta Okutani; Yasushi Asano; Tadashi Kawai

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
973 KB
Volume
7
Category
Article
ISSN
0887-8013

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✦ Synopsis

Protein 1 (Pl) is a low-molecular-weight protein recently isolated from the urine of patients with chronic renal failure. Its molecular weight is 14 kDa on sodium dodecyl sulfate polyacrylamide gel electrophoresis and pl 4.7 on isoelectric focusing. We purified this protein, characterized its physicochemical properties, and analyzed its amino acid sequences to show that it is probably identical to human lung Clara cell 10 kDa protein. Its monoclonal antibody was prepared, and a reliable enzyme-linked immunosorbent assay employing the sandwich method was developed and used to investigate distribution and variation in concentration of P i in

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