𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Investigation of dye/protein interaction and its application to enzyme purification

✍ Scribed by Antanas A. Glemža

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
508 KB
Volume
3
Category
Article
ISSN
0952-3499

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

In this review the results of the interaction of the active dyes used in the USSR textile industry with microbial enzymes and blood serum proteins are discussed. The complexity of dye/protein interaction and the dependence of this interaction on different factors is demonstrated. Some practical aspects of the use of dye containing sorbents are presented and discussed. Their suitability for RNA ligase and DNA ligase, acetate kinase, alcohol dehydrogenase, lactate dehydrogenase and glucose‐6‐phosphate dehydrogenase purification and blood serum protein fractionation is demonstrated.

📜 SIMILAR VOLUMES

Chemiluminescent assay of various enzyme
Chemiluminescent assay of various enzyme activites and its application to enzyme immunoassays
✍ Maeda, Masako ;Arakawa, Hidetoshi ;Tsuji, Akio 📂 Article 📅 1989 🏛 John Wiley and Sons ⚖ 602 KB

A highly sensitive chemiluminescent assay for NAD(P)H have been developed. The principle of the method is as follows; NAD(P)H reduces molecular oxygen t o superoxide anion (0;) and hydrogen peroxide (H202) in the presence of I-methoxy-5-methylphenazinium methyl sulphate (I-MPMS) as electron mediator

Electrochemical Studies on the Interacti
Electrochemical Studies on the Interaction of Protein with Beryllon III and Its Analytical Application
✍ Sun Wei; Jiao Kui; Wang Xueliang; Lu Lude 📂 Article 📅 2005 🏛 John Wiley and Sons 🌐 English ⚖ 112 KB 👁 1 views

A voltammetric study of the interaction of 5-(4-diethylamino-2-hydroxyphenylazo)-4-hydroxynaphthalene-2,7disulfonic acid (beryllon III) with protein at the mercury electrode in Britton-Robinson (B-R) buffer solution is described in this paper. In pH 3.5 B-R buffer solution, beryllon III has a well-d