Artificial immunoglobulin G-binding protein mimetic to staphylococcal protein A: Its production and application to affinity purification of immunoglobulin G

Exploring variation in binding of Protei

Exploring variation in binding of Protein A and Protein G to immunoglobulin type G by isothermal titration calorimetry

✍ Line Naomi Lund; Trine Christensen; Eric Toone; Gunnar Houen; Arne Staby; Phaedr 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 403 KB

Bacterial Protein A (PrtA) and Protein G (PrtG) are widely used for affinity purification of antibodies. An understanding of how PrtA and PrtG bind to different isotypes of immunoglobulin type G (IgG) and to their corresponding Fc fragments is essential for the development of PrtA and PrtG mimetic l