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Protamine sulfate precipitation: A new assay for the Ah receptor

โœ Scribed by Michael S. Denison; Jay Fine; Christopher F. Wilkinson

Book ID
102987159
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
745 KB
Volume
142
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

The ability of protamine sulfate to effect the quantitative precipitation of 2,3,7,8-[3H]tetrachlorodibenzo-p-dioxin (TCDD):Ah receptor complexes from rat liver cytosol has been developed into a new assay for the identification, quantitation, and characterization of the Ah receptor. The method is reliable, uncomplicated, and rapid, and can be applied to large numbers of samples. The major advantage of the assay is that protamine sulfate appears to selectively precipitate the Ah receptor protein and does not precipitate a number of other proteins that bind [3H]TCDD nonspecifically.

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โœ Marc Lippman; Karen Huff ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 514 KB

Separate receptors for estrogen and androgen are demonstrated in cells from metastic human breast cancer. By criteria of binding affinity, number of binding sites, and specificity of the receptor for different steroids, the receptors are shown to be distinguishable. The protamine sulfate receptor as