Prostaglandin synthesis by rat isolated glomeruli and glomerular cultured cells
โ Scribed by J. Sraer; J. Foidart; D. Chansel; P. Mahieu; R. Ardaillou
- Book ID
- 107770187
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 619 KB
- Volume
- 12
- Category
- Article
- ISSN
- 0020-711X
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## MATERIALS AND METHODS isoprostanes resulting from lipid peroxidation in Materials. [ 3 H]PGE 2 (120 Ci/mmol), [ 3 H]PGF 2a (168 Ci/mmol), and hepatocytes. F 2 -isoprostane release was detected by im-[ 3 H]L-leucine (5 Ci/mmol) were purchased from New England Numunoassay. Conventional cultures o
The synthesis of metabolically labeled proteoglycans and glycosaminoglycans from medium, cell layer and substrate attached material by rat glomerular mesangial cells in culture was characterized. The cellular localization of the labeled proteoglycans and glycosaminoglycans was determined by treating