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Proteoglycan and glycosaminoglycan synthesis by cultured rat mesangial cells

โœ Scribed by Gerald C. Groggel; Peter Hovingh; Alfred Linker

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
627 KB
Volume
147
Category
Article
ISSN
0021-9541

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โœฆ Synopsis

The synthesis of metabolically labeled proteoglycans and glycosaminoglycans from medium, cell layer and substrate attached material by rat glomerular mesangial cells in culture was characterized. The cellular localization of the labeled proteoglycans and glycosaminoglycans was determined by treating the cells with Flavobacterial heparinase. Of the total sulfated glycosaminoglycans, 33% were heparan sulfate; 55% of the cell layer material was heparan sulfate; 80% of sulfated proteins in the medium were chondroitin sulfateiderrnatan sulfate. Putative glycosaminoglycan free chains of heparan sulfate and chondroitin sulfate were found in both the medium and cell layer; 95% of total proteoglycans and most (90%) of the putative heparan sulfate free chains were removed from the cell layer by the heparinase, whereas only 50% of the chondroitin sulfate and 25% of dermatan sulfate were removed. Large amounts of hyaluronic acid labeled with DC), Irving Scientific (Santa Ana, CA), Sigma C 8" em-

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