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Propylene oxide and epichlorohydrin induce DNA strand breaks in human diploid fibroblasts

✍ Scribed by Ada Kolman; Irina Spivak; Maria Näslund; Maria Dušinská; Björn Cedervall

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
109 KB
Volume
30
Category
Article
ISSN
0893-6692

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✦ Synopsis

The induction of DNA strand breaks in human dip-respectively). Using these two methods, the SSBs/ loid fibroblasts (VH-10) was demonstrated after in DSBs ratio was estimated to be 148 for PO and 44 vitro exposure with two carcinogenic epoxides, pro-for ECH. pylene oxide (PO) and epichlorohydrin (ECH). Alka-

The data obtained by the comet assay also demline DNA unwinding (ADU), pulsed field gel electro-onstrated a dose-dependent ability of PO and ECH phoresis (PFGE), and the comet assay were used to induce DNA damage. It was found that ECH was to measure DNA single-(SSBs) and double-strand about six times more effective as an inducer of DNA breaks (DSBs).

strand breaks compared to PO (200 and 32r100 A dose-dependent increase of DNA strand Mbp 01 rmMh 01 for ECH and PO, respectively). The breaks, measured by ADU, was observed in the SSBs/DSBs ratios calculated using comet assay and dose range 2.5-20 mMh of PO and 0.25-2 mMh PFGE data were 125 for ECH and 41 for PO. In of ECH. The dose-response of ECH was about five addition, ECH is about 10 times more toxic than times higher compared with that of PO (211 vs. 41 PO with respect to survival. These properties of ECH SSBsr100 Mbp 01 rmMh 01 ). The induction rates of can at least in part be explained by its higher chemi- DSBs, measured by PFGE, were found to be 18 cal reactivity connected with a higher rate of DNA times higher for ECH compared to PO (4.8 and alkylation.

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