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Propionate-regulated high-yield protein production in Escherichia coli

✍ Scribed by Sung Kuk Lee; Jay D. Keasling

Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
157 KB
Volume
93
Category
Article
ISSN
0006-3592

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✦ Synopsis

Abstract

A new expression system containing the Salmonella enterica prpBCDE promoter (P~prpB~) responsible for expression of the propionate catabolic genes (prp BCDE) and prpR encoding the positive regulator of this promoter has been developed and tested. The main features of the expression system compared to those based on the bacteriophage T7 promoter are low background expression and high induced expression in Escherichia coli strains BL21, BL21(DE3), MG1655, and W3110. In addition, propionate is an inexpensive, simple‐to‐use, nontoxic inducer that is attractive for large‐scale protein production. Hence, this new system is highly complementary to the widely used T7 promoter‐driven expression systems. © 2005 Wiley Periodicals, Inc.

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