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Properties of the reaction of GDP-mannose with endogenous polyisoprenylphosphates of liver membranes

✍ Scribed by Norah A. Butler; G. Wolf


Book ID
118311247
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
481 KB
Volume
68
Category
Article
ISSN
0006-291X

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The reaction catalyzed by bacterial GDP-mannose dehydratase (E.C. 4.2.1.47), the conversion of GDP-D-mannose to GDP-4-keto-6-deoxymannose (GDP-6-deoxy-D-lyxo-hexos-4-ulose), was studied with (6R)- and (6S)-GDP-D-[4-2H1,6-3H]mannose. Conversion of these stereospecifically labeled substrates in the pr

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## Abstract Membranes from Penicillium charlesii were separated into 6 fractions by sucrose density gradient ultracentrifugation. The least dense fraction (ρ = 1.1 g cm^βˆ’3^) contained GDP‐mannose: glycopeptide mannosyltransferases that transferred [^14^C] mannose onto mannopyranosyl‐(seryl/threoyl)