Several of the physical properties of calmodulin are changed significantly upon increasing the temperature from 25 to 37Β°C. Among the observed changes are a minor increase in intrinsic viscosity, a decrease in molecular ellipticity in the peptide region, the development of a difference spectrum in t
Properties of the aminotyrosine derivatives of calmodulin
β Scribed by R. F. Steiner; L. Marshall
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1985
- Tongue
- English
- Weight
- 812 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
Derivatives of calmodulin have been prepared in which tyrosine-99 and tyrosine-138 have been converted to their aminotyrosine derivatives. Significant changes in microenvironment occur in the presence of Ca2+. Both groups have a significant degree of mobility with respect to the protein matrix; this is Ca2+ dependent.
* From 2-component analysis. From 3-component analysis.
π SIMILAR VOLUMES
The interaction of cyclosporin A and dansyl cyclosporin A with bovine and wheat germ calmodulin has been monitored by measurements of induced changes in dansyl and bound toluidinyl naphthalene sulfonate fluorescence. The interaction is Ca2 +-dependent and 1 : 1. Measurements of the efficiency of rad