Properties of protein polymers as substratum for cell growth in vitro

✍ Scribed by A. Macieira-Coelho; L. Berumen; S. Avrameas

• Book ID: 102882417
• Publisher: John Wiley and Sons
• Year: 1974
• Tongue: English
• Weight: 916 KB
• Volume: 83
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040830308

✦ Synopsis

Abstract

The behavior of two established cell lines was found to vary when subcultivated on protein polymers covered with either negatively or positively charged substances. Results indicate that the influence on cell behavior is conditioned by the charge rather than by structural differences or degree of attachment of the substances to the polymer. Furthermore, the substratum seems to have just a physical effect at the cell membrane without any direct influence on cell metabolism.

Cells were also seeded on a calf serum polymer and it was found that serum loses its properties on cultivated cells when used as substratum.