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Propagation of human embryonic and induced pluripotent stem cells in an indirect co-culture system

✍ Scribed by Sheena Abraham; Steven D. Sheridan; Louise C. Laurent; Kelsey Albert; Christopher Stubban; Igor Ulitsky; Bradley Miller; Jeanne F. Loring; Raj R. Rao

Book ID
116299829
Publisher
Elsevier Science
Year
2010
Tongue
English
Weight
745 KB
Volume
393
Category
Article
ISSN
0006-291X

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## Abstract Human embryonic stem (hES) cells are typically maintained on mouse embryonic fibroblast (MEF) feeders or with MEF‐conditioned medium. However, these xenosupport systems greatly limit the therapeutic applications of hES cells because of the risk of cross‐transfer of animal pathogens. The