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Prooligonucleotide metabolism in a crude cell extract followed by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry

✍ Scribed by Thierry Lioux; Isabelle Lefebvre; Jean Jacques Vasseur; Jean Louis Imbach

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
64 KB
Volume
13
Category
Article
ISSN
0951-4198

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✦ Synopsis

Using matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOFMS) we studied the decomposition pathway of a prooligonucleotide during incubation in a crude cell extract. This study, involving no sample processing, except drop dailysis to remove salts, led us to identify more than thirty metabolites from several metabolic pathways.

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