Promotion of peripheral nerve growth by collagen scaffolds loaded with collagen-targeting human nerve growth factor-β

✍ Scribed by Wenjie Sun; Hang Lin; Bing Chen; Wenxue Zhao; Yannan Zhao; Jianwu Dai

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 336 KB
• Volume: 83A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.31417

✦ Synopsis

Abstract

Nerve growth factor (NGF) plays a critical role in neuronal development and regeneration. However, the lack of efficient NGF delivery system limits its clinical application. We reported that a peptide deduced from collagenase, TKKTLRT, fused with NGF‐β could develop a collagen based NGF targeting delivery system. Our results showed that this peptide could allow fused NGF‐β bind to collagen specifically. In addition, we found that the polypeptide could result in a 2.3‐fold increase in the expression level and a significant improvement of bioactivity of fused NGF‐β. In the in vivo function study, collagen membranes loaded with the collagen binding NGF enhanced the nerve growth. Thus, the targeting wound repair system could be important for the repair of peripheral nerve injury. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007