Vascularization and cellularization of collagen scaffolds incorporated with two different collagen-targeting human basic fibroblast growth factors
✍ Scribed by Wenxue Zhao; Bing Chen; Xiaodong Li; Hang Lin; Wenjie Sun; Yanhong Zhao; Bin Wang; Yannan Zhao; Qianqian Han; Jianwu Dai
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 480 KB
- Volume
- 82A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
To develop a collagen‐based wound targeting repair system, we introduced two collagen‐binding domains (CBDs) into the human basic fibroblast growth factor (bFGF). Three expression vectors were constructed: the first one (named V‐bFGF) contained bFGF and the CBD WREPSFCALS derived from von Willeband's factor (vWF); the second (named C‐bFGF) contained bFGF and the CBD TKKTLRT derived from collagenase; the third (named bFGF) was bFGF as a control. The recombinant proteins of V‐bFGF and C‐bFGF were demonstrated to retain both growth factor activity and collagen‐binding activity. We found that C‐bFGF possessed higher collagen‐binding ability than V‐bFGF. The targeted repair systems consisting of collagen scaffolds and CBD‐bFGFs were assembled in vitro and then implanted subcutaneously. Results showed that C‐bFGF promoted vascularization at the implanted sites more effectively than V‐bFGF. Histological analysis showed more cells migrated into collagen scaffolds incorporated with C‐bFGF than those with V‐bFGF. These data suggested that the higher collagen‐binding ability the CBD‐bFGF possessed, the more significant vascularization, and cellularization were observed. In summary, CBD‐bFGF/collagen system could be used as a targeted repair system with beneficial effects of the restriction of bFGF diffusion, the prolonging of bFGF activity, and the targeted promotion of vascularization and cellularization. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007