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Profilin Forms Tetramers That Bind to G-Actin

โœ Scribed by Michael Babich; Lisa R.P. Foti; Laura L. Sykaluk; Carl R. Clark


Book ID
115577842
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
79 KB
Volume
218
Category
Article
ISSN
0006-291X

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We have used a fluorescence assay to measure the binding of Acanthamoeba profilin to monomeric Acanthamoeba and rabbit skeletal muscle actin labeled on cysteine-374 with pyrene iodoacetamide. The wavelengths of the pyrene excitation and emission maxima are constant at 346 and 386 nm, but the fluores