Profile of energy metabolism in a murine hybridoma: Glucose and glutamine utilization

✍ Scribed by D. Petch; M. Butler

• Book ID: 102886411
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 653 KB
• Volume: 161
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041610110

✦ Synopsis

The antibody-secreting murine hybridoma, CC9C10, was grown in batch culture in a medium containing 20 m M glucose and 2 m M glutarnine. After 2 days of exponential growth, the glutamine content of the medium was completely depleted, whereas the glucose content was reduced to 60% of the original concentration. The glucose and glutamine metabolism was analyzed at midexponential phase by use of radioactively labelled substrates. Glycolysis accounted for the metabolism of most of the glucose utilized (>96%) with flux through the pentose phosphate pathway (3.6%) and the TCA cycle (0.6%) accounting for the remainder. Glutarnine was partially oxidised via glutaminolysis to alanine (55%), aspartate (3%), glutamate (4%), lactate (9%), and CO, (22%). Calculation of the theoretical ATP production from these pathways indicated that glucose could provide 59% and glutamine 41 % of the energy requirement of the cells.