Production, separation and purification of yeast invertase as a by-product of continuous ethanol fermentation

✍ Scribed by Errcheng Chan; Cheeshan S. Chen; C. S. Gong; Li Fu Chen

• Publisher: Springer
• Year: 1991
• Tongue: English
• Weight: 363 KB
• Volume: 36
• Category: Article
• ISSN: 1432-0614
• DOI: 10.1007/bf00164697

✦ Synopsis

A strain of Saccharomyces uvarum produced extracellular invertase in a chemostat reactor using a medium containing corn steep liquor and sugars. The production of yeast invertase increased with increase in corn steep liquor concentration. The production rate of invertase was maximal at a dilution rate of 0.75 h -1. The production rate of invertase was found to be affected by the type of sugar substrate and fermentation temperature. The invertase in the crude broth could be purified by one-step DEAE chromatography. An 84% enzyme recovery with ninefold purification and 30-fold concentration could be achieved using this simple isolation procedure.